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Genetic diversity of the
monomeric prolamins and hordein in hulless barley genotypes
and their relation with agronomical traits
R. Eshghi* and E.
Akhundova
Baku State
University, Baku, Azerbaijan.
*Corresponding author. E-mail:
rohameshghi@yahoo.com.
Tel.: +99450-7164-789.
Accepted
3 February, 2009 |
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In order
to compare polymorphism resulting from monomeric prolamine
and hordein, 63 genotypes of hulless barley from ICARDA were
investigated. Also, to compare the patterns observed in the
analyses of storage proteins and their relation with
morphologic and phenologic traits, 20 agromorphological
traits were recorded. In the analysis of the hordeins, no
polymorphism was observed in the area D hordein. However, 10
patterns in the area C hordein, and 13 patterns in the area
B hordein were observed; and in total 32 bands and 32
patterns were observed. The average genetic diversity index
for these proteins was calculated as H = 0.866. In the
analysis of the monomeric prolamins, which was performed
with the Acid-PAGE method, 15, 9, 24, and 20 patterns were
observed for the ω, γ, β, and α areas, respectively. The
average of the genetic diversity index for these proteins
was H = 0.889, and in total 33 bands as well as 57 patterns
were observed. In the analysis of the monomeric prolamins,
51 genotype having unique patterns were identifiable while
in the analysis of the hordeins, only 18 samples were
identified. With regard to the fact that monomeric prolamins
enjoy a greater diversity than hordeins and are more
powerful in identifying samples and regarding the simple, in
addition to the low cost of conducting the analysis, it can
be used in a variety of genetic studies such as genetic
diversity assessment, identifying genotypes and determining
the phylogenic relations in barley. In the analysis of
hordeins, one pattern was found among the patterns of area B
hordein that was related to the traits of days to heading
and days to maturity.
Key
words:
Genetic
diversity, monomeric prolamins, hordein, hulless barley. |
