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Laboratory scale
production of the human recombinant iduronate 2-sulfate
sulfatase-Like from Pichia pastoris
Henry A. Córdoba-Ruiz1, Raúl A. Poutou-Piñales1,3,
Olga Y. Echeverri-Peña1, Néstor A.
Algecira-Enciso2, Patricia Landázuri1,4,
Homero Sáenz1,5 and Luis A. Barrera-Avellaneda1*
1Instituto
de Errores Innatos del Metabolismo, Facultad de Ciencias,
Pontificia Universidad Javeriana. Bogotá D.C., Colombia.
2Universidad
Nacional de Colombia.
3Laboratorio
de Biotecnología Aplicada, Grupo de Biotecnología Ambiental
e Industrial, Departamento de Microbiología. Facultad de
Ciencias, Pontificia Universidad Javeriana. Bogotá D.C.,
Colombia.
4Laboratorio
de Bioquímica y Genética. Facultad de las Ciencias de la
Salud Universidad del Quindío, Armenia, Colombia.
5Unidad
de Biología Celular y Microscopía, Decanato de Ciencias de
la Salud, Universidad Centroccidental Lisandro Alvarado,
Barquisimeto, Venezuela.
*Corresponding author. E-mail:
abarrera@javeriana.edu.co. Fax: 57-1- 338-45-48.
Accepted
30 March, 2009 |
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Clone
IDS28 of the yeast Pichia pastoris expressing the
human iduronate 2-sulfate sulfatase-Like (hIDS-Like)
was employed for low-scale production of the recombinant
enzyme in a saline culture media without phosphate. The
biological activity found was between 7.3 and 29.5 nmol h-1
mg-1 of total protein. It is about 1.73 to 7
times higher than the result obtained with the same clone in
shake flask culture.
Key
words:
Pichia pastoris, iduronate 2-sulfate sulfatase, monod
model, Hunter syndrome, methanol induction. |
