|
In vitro
propagation and free radical studies of Smilax zeylanica
Vent
R. Thirugnanasampandan*, V. N. Mutharaian and V. Narmatha
Bai
Department
of Botany, Bharathiar University, Coimbatore, Tamil Nadu,
India.
*Corresponding author. E-mail:
thirugnanasampandan@yahoo.co.in.
Abbreviations: BA,
6-benzyl adenine; KIN, kinetin; IAA, indole-3-
acetic acid; IBA, indole-3-butyric acid;
NAA,
naphthalene acetic acid; L-GLU, L-glutamine;
DPPH,
1,
1-diphenyl-2-picrylhydrazyl; TBA, thiobarbituric
acid; MDA, malonaldehyde.
Accepted
2 January, 2008 |
|
A method of micropropagation through multiple shoots
formation from nodal segments of Smilax zeylanica
Vent. has been developed. The nodal explants were cultured
on half strength MS medium containing BA (0.5 mg/l) and IAA
(1 mg/l) with activated charcoal (100 mg/100 ml) produced
single shoot within 7-10 days. Shoots were multiplied by
using nodal segments of in vitro regenerated shoot in
modified half strength MS medium supplemented with KIN (2
mg/l), L-Glu (0.5 mg/l) and activated charcoal (100 mg/100
ml). Two shoots were formed. Rooting
of the microshoots was achieved in half strength MS medium
fortified with IBA (1 mg/l) within three weeks. The rooted
plantlets transferred to potting medium containing
vermiculite, sand and coir pith (1:1:1). Survival of the
plantlets under ex vitro condition was 70%. In
vitro antioxidant activities of leaf and stem extracts
were performed. Among the extracts tested, ethanol extract
of the stem showed maximum DPPH (52.361%) scavenging
activity and chloroform extract of stem inhibited hydroxyl
radical mediated linoleic acid oxidation up to 50.87%.
Ethanol extract of leaf showed maximum reducing power of
0.53. The total free phenolics were found to be 293.3 μg in
the ethanol extract of leaf.
Key
words:
Smilax zeylanica, nodal culture, rooting, antioxidant
activity. |
