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Shoot organogenesis in
oleaster (Elaeagnus angustifolia L.)
Omid Karami* and Khosro Piri
Department
of Biotechnology, Faculty of Agriculture, Bu-Ali Sina
University, Hamadan, Iran.
*Corresponding
author. E-mail:
hiva@basu.ac.ir. Tel: #9183163372. Fax: #8114227021.
Abbreviations: BA,
6-Benzyladenine; 2,4-D, 2,4-Dichlorophenoxyacetic
acid; MS, Murashige and Skoog (1962) basal medium;
NAA, α-naphthalene acetic accid; IBA,
indole-3-butyric acid; TDZ, N-phenyl-N0
(1,2,3-thidiazol-5-yl) urea.
Accepted
18 February, 2008 |
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Efficient plant regeneration through organogenesis was
achieved from callus cultures derived from leaf explants of
Elaeagnus angustifolia.
Calli were obtained on MS medai containing
3% sucrose and different
concentrations of TDZ (0.5, 1, 2,
3, 4 and 6 mg/l). Maximum percentage response for
callus formation was 85% on MS medium supplemented with 2
mg/l of TDZ. Shoot organogenesis was achieved after transfer
of calli to MS media with 3%
sucrose and different concentrations of
BA (0.5, 1, 2, 3, 4 and 6 mg/l) and kinetin (0.5, 1, 2, 3, 4
and 6 mg/l). Maximum organogenesis was obtained with
2 mg/l of BA. Rooting of the
shoots was achieved on MS supplemented with 0.2 mg/l of IBA.
Regenerated plantlets were acclimatized and successfully
transplanted to soil.
Key
words:
shoot organogenesis, callus, oleaster. |
