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Determination of
the relationship among Old World Lupin (Lupinus sp.)
species using RAPD and ISSR markers
Mustafa
Yorgancilar*, Mehmet Babaoglu,
Erdogan E. Hakki and Emine
Atalay
Department of Field Crops, Faculty of Agriculture,
University of Selçuk,
42075 Kampus, Konya, Turkiye.
*Corresponding author. E-mail:
myorg@selcuk.edu.tr.
Accepted 29 June, 2009 |
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Randomly Amplified Polymorphic DNA (RAPD) and Inter
Simple Sequence Repeat (ISSR) markers were used to determine
the genetic relationships among 20 Old World lupin genotypes
from 3 lupin species (Lupinus albus, L. angustifolius, L.
luteus) using 15 primers in each case. Polymorphism was
observed in reactions with 9 out of 15 tested RAPD primers,
and 11 out of 15 tested ISSR primers. Relationship was
determined on the basis of polymorphic products analysis
which was shown in the form of dendrograms by UPGMA method. Both RAPD and ISSR markers worked well to identify
distinct grouping among three species and among genotypes
within a species. The
parallel use of both data set seems to allow precise
estimation of cultivars relationship and diminishing
mistakes connected with method’s technical limitations.
Such marker systems may potentially be used in the development of new
lupins cultivars.
Key words:
White lupin, genetic diversity,
RAPD, ISSR. |
