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Assessment of
hairy roots induction in Solenostemon scutellarioides
leaves by different strains of Agrobacterium rhizogenes
Saleh, N. M.*
and Thuc, L. V.
Department of Cell and Molecular Biology, Faculty of
Biotechnology and Biomolecular Sciences, University Putra
Malaysia, 43400 Serdang Selangor Darul Ehsan, Malaysia.
*Corresponding author.
E-mail:
norihanms@biotech.upm.edu.my.
Tel.: 603-89468357. Fax: 603 89467510.
Accepted 2 June, 2009 |
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Hairy roots of Solenostemon scutellarioides
were induced by inoculation of leaf explants with
Agrobacterium rhizogenes strains TR 105, LBA 9402, 8196
and ATCC 15834. These strains showed different abilities to
induce hairy root formation on the leaf explants. Assessment
of the plant’s susceptibility to the different strains of
A. rhizogenes showed that strains ATCC 15834, TR 105,
LBA 9402, and 8196 produced 56.3, 25.5, 21.5 and 13.8%
transformation efficiencies, respectively. Acetosyringone
was found to be useful for the enhancement of hairy roots
formation in S. scutellarioides.
Key words:
Solenostemon scutellarioides, hairy roots,
acetosyringone, Agrobacterium rhizogenes, Labiatae. |
