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Full Length Research Paper
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Polypeptides associated
with the induction and maturation of direct somatic
embryogenesis in peanut (Arachis hypogaea L.)
A. Roja Rani1*,
P. Venkateswarlu2,
Arun Kumar2, G. Padmaja2 and
Nimala Baburao3
1Department
of Genetics, Osmania University, Hyderabad 500 007, India.
2School
of Life Sciences, University of Hyderabad, Hyderabad 500
046, India.
3Department
of Botany, Osmania University College for Women, Hyderabad
500095, India.
*Corresponding author. E-mail:
anupalli_rr@yahoo.com.
Tel: 9440739631.
Accepted 23
July, 2009 |
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Abstract |
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Analysis of protein patterns in mature zygotic embryo axes
and somatic embryos by two-dimensional gel electrophoresis
revealed that protein of spot no. E4 (37 kDa, pI
9.5) and E5 (37 kDa, pI 9.8) are present only in
the 6 day old culture indicating their importance for
induction of somatic embryogenesis. Proteins of spot no. E1
(53kDa, pI 8.2), E2 (53kDa, pI 8.3) and E3
(37 kDa, pI 9.3) expressed in 6 day-old immature
zygotic embryo axis cultures were also present in globular
staged somatic embryos and these proteins are possibly
associated with early stages of somatic embryogenesis.
Proteins of 65 kDa separated into different spots of C1,
C2, C3, C4 with pI values
ranging from 6.3-9.6 on two-dimensional gel are present only
in zygotic embryos. Proteins of C2 (65 kDa, pI
7.5), C8 (22 kDa, pI 7.8), C10 (20 kDa,
pI 5.3) C11 (20 kDa, pI 5.5) were specifically
expressed in mature zygotic embryo axes and are possibly
associated with storage or late embryonic proteins. A few
proteins showed differential expression during various
stages of somatic embryos and many proteins that expressed
in cotyledonary somatic embryos and were also present in
immature and mature zygotic embryo axes.
Key
words:
Two-dimensional gel electrophoresis, somatic embryos. |
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