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Changes in protein
composition and protein phosphorylation during somatic
embryogenesis and plant regeneration in peanut (Arachis
hypogaea L.)
A. Roja Rani1*,
P. Venkateswarlu1 , G. Padmaja2, K.
Venkatesh1 and Nimala Baburao3
1Department
of Genetics, Osmania University, Hyderabad 500 007, India.
2School
of Life Sciences, University of Hyderabad, Hyderabad 500
046, India.
3Department
of Botany, Osmania University College for Women, Hyderabad
500095, India.
*Corresponding author.
E- mail: anupalli_rr@yahoo.com.
Tel.: 9440739631.
Accepted 22
May, 2009 |
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Changes
in protein profiles and protein phosphorylation were studied
in various stages of germinating somatic and zygotic
embryos. Many proteins, which were expressed in cotyledonary
stage somatic embryos, were also present in the zygotic
embryos obtained from mature dry seed. The intensity of 22
kDa protein was much higher in zygotic embryos in comparison
to cotyledonary somatic embryos. Proteins of 55, 53, 27 and
25 kDa did not vary in expression during different stages of
germination of somatic and zygotic embryos. There was rapid
accumulation of 28 kda protein in germinating zygotic and
somatic embryos at 3rd stage and persisted during
the subsequent stages of germination indicating the
emergence of radical and plumule. A clear difference was
observed in the patterns of protein phosphorylation in
germinating somatic and zygotic embryos. A 50 kDa protein
was heavily phosphorylated in mature zygotic embryos at 2nd
stage of germination and disappeared in 3rd stage
of germination. Protiens of 50 and 43 kDa proteins showed
enhanced phosphorylation up to 3rd stage and
drastic reduction was observed at 4th stage. In
addition, phosphorylation of 68 and 65 kDa specifically
appeared in 3rd stage of germinating somatic
embryos and were not observed during any germinating stages
of zygotic embryos.
Key words:
Protein analysis, sodium dodecyl sulphate polyacrylamide gel
electrophoresis, protein phosphorylation, zygotic embryo
axis. |
