Full Length Research Paper

Genetic diversity among varieties and wild species accessions of pea (Pisum sativum L.)  based on SSR markers

J.  Nasiri¹, A. Haghnazari²* and J. Saba²

¹Department of Plant Breeding, College of Agriculture, Zanjan University, Zanjan- Iran.

²Department of Genetics and Plant Breeding, College of Agriculture, Zanjan University, Zanjan- Iran.

*Corresponding author. E-mail: ahaghnazari@gmail.com. Tel.: +982415152465. Fax:  +982415152546.

Accepted 13 April, 2009

   Abstract

To assess the genetic relations in Pisum genus and to examine putative duplicate accessions, 20 pea varieties (Pisum sativum L.) with 57 accessions from wild Pisum species fulvum, subspecies (subsp.) asiaticum, elatius, thebaicum, abyssinicum, transcaucasicum and arvense were analyzed using 10 out of 20 microsatellite primer pairs. We genotyped all accessions. In total, 59 alleles were identified in whole collection. The maximum number of alleles (8 alleles) was obtained from the PEACPLHPP, AF004843, and AA43090 loci.  The maximum number of private alleles (4) in the wild collection was detected in AF004843 locus but in the cultivar collection, it was detected in AA430902 and PSBLOX13.2 loci. Cluster analysis and principal coordinate analysis located accessions in 3 groups and cultivated varieties were obviously separated from the wild accessions. Analysis of molecular variance (AMOVA) revealed that the intergroups component of variance (29%) is lower than the intragroups component of variance (71%). The lowest value of genetic differentiation (Φ_PT = 0.27) of pair wise collections between wild and variety collections, was detected in ssp. elatius. Assignment test on the basis of log-likelihood to estimate the likelihood that an individual belongs to a given group, showed that 96% of accessions being assigned correctly to their groups. This study showed that genetic probability profiles of accessions can corroborate clustering analyses while providing additional information as a powerful tool for assigning accessions into their related groups.

Key words: Genetic diversity, SSR, Pisum, cluster analysis, assignment test.