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Molecular
cloning, characterization and functional analysis of a
3-hydroxy-3- methylglutaryl coenzyme A reductase gene from
Jatropha curcas
Juan Lin*,
Yuanjie Jin, Mingqi Zhou, Xin Zhou and Jinya Wang
State Key Laboratory of Genetic Engineering, School of Life
Sciences, Morgan-Tan International Center for Life Sciences,
Fudan-SJTU-Nottingham Plant Biotechnology R and D Center,
Fudan University, Shanghai 200433, China.
*Corresponding author. E-mail:
linjuan@fudan.edu.cn.
Tel.: +86-21-65642425.
Fax: +86-21-65642425.
Accepted 15 May, 2009 |
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3-Hydroxy-3-methylglutaryl coenzyme A reductase (HMGR,
EC: 1.1.1.34) catalyzes the first committed step in
mevalonic acid (MVA) pathway for biosynthesis of
isoprenoids. The full-length cDNA encoding HMGR was isolated
from Jatropha curcas for the first time (designated
as JcHMGR), which contained a 1950 bp ORF encoding 584 amino
acids. The JcHMGR genomic DNA sequence was also obtained,
revealing JcHMGR had 4 exons and 3 introns. The deduced
JcHMGR protein showed high identity to other plant HMGRs and
contained 2 transmembrane domains and a catalytic domain.
The potential significance of JcHMGR gene was also
discussed.
Key words: Jatropha curcas, RACE, 3-hydroxy-3-methylglutaryl coenzyme A
reductase, color complementation assay. |
