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Allele and genotype
frequencies of -βlactoglobulin gene in Iranian Najdi cattle
and buffalo populations using PCR-RFLP
K. Karimi*, M. T. Beigi Nasiri, J. Fayyazi, K.
H. Mirzadeh and H. Roushanfekr
Department
of Animal Science, Ramin Agricultural University, Ahvaz,
Iran.
*Corresponding author. E-mail:
karim.karimi81@gmail.com.
Phone:
0711-7500790. Fax: 06123224351.
Accepted 2
December, 2008 |
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In modern programmes of animal breeding, the polymorphism of
the milk proteins can be used as marker systems. â-Lactoglobulin
is the major milk whey protein in the ruminants. Studies
have indicated that this protein is polymorphic in the many
breeds of cattle. This is the result of a single base pair
substitution in the â-lactoglobulin gene that also give rise
to the Hae III restriction fragment length
polymorphism. The aim of this work was to analyze the
genotype distribution of â-lactoglobulin in Iranian Najdi
cattle and buffalo. Blood samples were supplied from 80
Najdi cattle and 80 buffalo from different cities of
Khouzestan province. Polymerase chain reaction was applied
to amplification of a 247 bp fragment of exon and intron IV
of bovine â-lactoglobulin gene. The Hae III enzyme
was used for restriction of the PCR products. The digested
products were separated by electrophoresis on 2.5% agarose
gel. The allele B of â-Lactoglobulin occurred at a higher
frequency than the allele A in both Najdi cattle and
buffalo. The genotype frequencies of AA, AB, and BB in Najdi
cattle and buffalo were 0, 0.175, 0.825 and 0.04, 0.3, 0.66
respectively. Frequencies of A and B alleles were 0.0875 and
0.9125, and 0.1875 and 0.8125 in Najdi cattle and buffalo,
respectively. The deviation from Hardy-Weinberg equilibrium
was not detected in any of those animals. Detection of
heterozygosity indicator evidence on low level of
heterozygosity and genetic variability in both populations.
Key
words:
Polymorphism, â-lactoglobulin, PCR-RFLP, Najdi cattle,
Buffalo. |
