|
Full Length Research Paper
|
|
Synchronous production of
conidial powder of several fungal biocontrol agents in
series fermentation chamber system
Chun Chen1,2*,
Zhengliang Wang2, Sudan Ye3 and
Mingguang Feng2
1College
of Life Sciences, China Jiliang University, Hangzhou,
Zhejiang 310018, People’s Republic of China.
2Institute
of Microbiology, College of Life Sciences, Zhejiang
University, Hangzhou, Zhejiang, 310058, People’s Republic of
China.
3Zhejiang
Economic and Trade Polytechnic, Hangzhou 310018, People’s
Republic of China.
*Corresponding author. E-mail:
aspringjohn@yahoo.com.
Tel.: 86-571-86835704.
Accepted 29
June, 2009 |
|
|
|
Abstract |
|
|
|
A series
solid-state fermentation (SSF)
chamber system was designed to produce superior conidial
powder of several fungal biocontrol agents. The
synchronous
conidia production of six fungal biocontrol candidates in three genera including Metarhizium
flavoviride ARSEF
5628,
M. anisopliae ARSEF
456, Beauveria bassiana ARSEF
734
and ARSEF 2860, Paecilomyces fumosoroseus ARSEF
6206
and ARSEF 3843 were assessed for the feasibility of chamber
system. The conidial powder of those strains harvested from
5-kg rice cultures in a chamber weighed 147.1, 137.5, 101.2,
89.2, 55.0 and 40.0 g, and obtained 2.2
´
1012, 2.7
´
1012, 5.4
´ 1012, 4.6
´
1012, 1.8
´
1012, 1.3
´ 1012 conidia kg-1
rice, respectively. Experimental data from the new series
chamber system were obviously better than those of previous
reports. Moreover, conidial
viability of those isolates was up to 95% entirely. Results
suggest that the chamber system have the capability to meet
the quick and simple biocontrol evaluation activities such
as biological characteristic of various biocontrol agents,
from synchronous production to multiplicate trials.
Key
words:
Aerial conidia, fungal pathogens, synchronous production,
series fermentation chamber. |
|
