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Potential
phytate-degrading enzyme producing bacteria isolated from
Malaysian maize plantation
Anis Shobirin M.
H.1*, A. Farouk2 and R. Greiner3
1Department
of Food Technology, Faculty of Science and Technology,
Universiti Putra Malaysia, 43400, UPM, Serdang, Selangor.
2Faculty
of Science, University of Taif,
Taif, Al-Hawiya 888, Saudi Arabia.
3Divisão
de Engenharia de Bioprocessos e Biotecnologia, Universidade
Federal do Paraná, Centro Politécnico, Jardim das Américas,
81531-970 - Curitiba, PR, Brazil.
*Corresponding author. E-mail:
anisshobirin@gmail.com.
Tel: 60389468352.
Fax: 60389423552.
Accepted 21 May, 2009 |
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Phytases catalyze the hydrolysis of phosphomonoester
bonds in phytate, thereby releasing lower forms of
myo-inositol phosphates and inorganic phosphate. Phytase
enzyme preparations have a wide range of applications in
animal and human nutrition. The addition of
phytate-degrading enzyme can improve the nutritional value
of plant-based foods by enhancing protein digestibility and
mineral availability through phytate hydrolysis during
digestion in stomach or during food processing. 30 strains
of potential phytate-degrading enzymes bacteria isolated
from Malaysian maize plantation were cultivated in Luria
Bertani (LB) and Luria Bertani + Rice Bran (LBRB) media for
5 days and were analyzed for phytase activity. The 6 strains
with highest activity were chosen for species
identification. Two set of broad-range 16S rRNA PCR primers
were used for genotypic identification. ASUIA279 was the
strain that had has the highest phytase activity in LBRB
followed by ASUIA271, ASUIA138, ASUIA260, ASUIA243 and
ASUIA30. The genotypic technique revealed Pantoea
stewartii ASUIA271, Enterobacter sakazakii
ASUIA279, Bacillus cereus ASUIA260, Bacillus
subtilis ASUIA243, P. stewartii ASUIA138 and
B. cereus ASUIA30.
Key words:
Bacterial phytase, rice bran, production, genotypic. |
