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Analysis of
genetic relationships of mulberry (Morus L.)
germplasm using sequence-related amplified polymorphism (SRAP)
markers
Weiguo Zhao1,2,
Rongjun Fang2, Yile Pan2, Yonghua Yang3,
Jong-Wook Chung4, Il-Min Chung1*, and
Yong-Jin Park4*
1Department
of Applied Bioscience, Konkuk University, Seoul 143-701,
Republic of Korea.
2Jiangsu
University of Science and Technology, Sericultural Research
Institute, Chinese Academy of Agricultural Sciences,
Zhenjiang 212018, China.
3School
of Life Sciences, Nanjing University, Nanjing 210093, China.
4Department
of Plant Resources, College of Industrial Science, Kongju
National University, Yesan 340-802, Republic of Korea.
*Corresponding authors. E-mail:
yjpark@kongju.ac.kr
or imcim@konkuk.ac.kr.
Accepted 12 May, 2009 |
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Sequence-related amplified polymorphism (SRAP) is a novel
molecular marker technique designed to amplify open reading
frames (ORFs). Here, for the first time, SRAP was used to
identify mulberry germplasm. Twenty-three mulberry
accessions were screened using SRAP techniques, with 12
primer combinations selected for their reproducibility and
polymorphism. Out of 83 PCR fragments scored, 59 (71.1%)
were polymorphic, with an average of 4.9 polymorphic bands
and 6.9 bands per primer combination. Mean gene diversity
and PIC were 0.1611 and 0.1353, respectively. The genetic
similarity coefficient ranged from 0.6905 to 0.9524, with an
average of 0.8330. The phylogenetic tree was obtained using
the UPGMA method using the total number of amplified SRAP
fragments.
The results from cluster analysis were in general agreement
with our morphologic classification.
Key words:
Mulberry, molecular marker, genetic diversity, SRAP. |
