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Isolation and
purification of alkaline keratinase from Bacillus sp.
50-3
Bin Zhang1*,
Zhong-wei Sun1, Dan-Dan Jiang2 and
Tian-Gui Niu1
1College
of Food Science and Nutritional Engineering, CAU, Beijing,
China.
2Beijing
Homeport Food Ingredients Trading Company, Beijing, China.
*Corresponding author. E-mail:
niutiangui@163.com. Tel: +86-10-62737045, Fax:
+86-10-62737045.
Accepted 15 May, 2009 |
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A new alkaline keratinase extracted from Bacillus sp.
50-3 was isolated and purified in this study. Solid ammonium
sulfate was selected to precipitate the enzyme. Its proper
adding mass was also determined. Through solid ammonium
sulfate precipitation and liquid chromatography via the
DEAE-Sephadex-A50 column using with azokeratin as a
substrate, 17.7-fold purification with a yield of 46.5% was
obtained. The purification effect was determined through
SDS-PAGE, and the molecular weight of the enzyme was
found at 27 423 Da by the
MALDI-TOF-MS.
Its simple purification step and high yield using a cheap
medium attest to the great biotechnological potential of
keratinase, especially in environment protection and in
recycling valuable materials from wastes.
Key words:
Bacillus, chicken feathers, environment protection,
keratinase, purification. |
