Full Length Research Paper

An AFLP marker linked to turnip mosaic virus resistance gene in pak-choi

Wang Xinhua¹, Chen Huoying¹*, Zhu Yuying² and Hou Ruixian²

¹School of Agriculture and Biology, Shanghai Jiaotong University, 800# Dongchuan Road, Shanghai, 200240, PR China.

²Protected Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, 201106, PR China.

*Corresponding author. E-mail: Chhy@sjtu.edu.cn. Tel./Fax: +86 21 34206934.

Accepted 12 May, 2009

Pak-choi is one of the most important vegetable crops in China. Turnip mosaic virus (TuMV) is one of its main pathogen. Screening the molecular marker linked to the TuMV resistance gene is an efficient method to improve pak-choi breeding. In this paper, a dominant gene, TuRBCH01, has been mapped. 180 F₂ individuals were inoculated with TuMV-C5 and tested by direct ELISA. The 3:1 ratio of F₂ hybrids segregation proved a single dominant allele for TuMV resistance. Amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) method were used to study the F₂ population. An AFLP marker (EccMctt3) linked to TuMV resistance gene with 7.8cM map distance was identified.

Key words: Pak-choi, TuMV resistance, AFLP, BSA.