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Influence of phytohormones
and medium on the shoot regeneration from leaf of Swertia
chirata Buch.-Ham. Ex wall. in vitro
Li Wang1,2,3,
Lizhe An1, Yanping Hu2, Lixin Wei2
and Yi Li2*
1Cold
and Arid Regions Environmental and Engineering Research
Institute, the Chinese Academy of Science, Lanzhou 730000,
China.
2Key
Laboratory of Adaptation and Evolution of Plateau Biota
Northwest Institute of Plateau Biology, the Chinese Academy
of Science, Xining 810001, China.
3Graduate
University of Chinese Academy of Science, Beijing 100049,
China.
*Corresponding author. E-mail:
liyi@nwipb.ac.cn.
Tel.: +86-9716101454.
Fax: +86-971-6101454.
Abbreviations:
ANOVA, Analysis of variance; 6-BA,
6-benzyladenine; NAA, a-naphthaleneacetic acid; MS,
Murashige and Skoog basal medium; B5, B5 basal
medium; 190-2, 190-2 basal medium.
Accepted
14 May, 2009 |
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A procedure
for in vitro shoot regeneration was formed from
leaves derived from the field grown Swertia chirata
and the effects of phytohormones and medium on the
regeneration of shoot were tested by an orthogonal
experiments. The best result was obtained in the 190-2
medium with 13.32
mM
6-benzylaminopurine and 0.54
mM
α-naphthaleneacetic acid. The result of orthogonal analysis
of variance showed that 6-benzylaminopurine and α-naphthaleneacetic
acid significantly affected on the shoot regeneration. The
propagation rate was 7.75 ± 3.46 when the shoots were
cultured on the multiplication medium. The highest rate of
shoots was 96.50% on the medium with 5.40
mM
α-naphthaleneacetic acid. Histological studies showed shoot
regeneration took place by somatic embryogenesis.
Key
words:
Swertia chirata, adventitious shoots, histology, an
orthogonal test, leaves. |
