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Culture
conditions for the production of a tannase of Aspergillus
tamarii IMI388810 (B)
Enemuor, S.
C.1* and Odibo, F. J. C.2
1Department
of Microbiology, Kogi State University, PMB 1008, Anyigba,
Kogi State, Nigeria.
2Department
of Applied Microbiology and Brewing, Nnamdi Azikiwe
University, PMB 5025, Awka, Nigeria.
*Corresponding author. E-mail:
enemec2001@yahoo.com.
Accepted 13 April, 2009 |
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Aspergillus tamarii
IMI388810 (B) a tannic acid degrading fungus was isolated
from soil inundated by effluent of a tannery at Oji River
local Government Area of Enugu State, Nigeria. It was
identified by CABI Bioscience, United Kingdom as A.
tamarii with accession number of IMI 388810 (B). This
fungus produced tannase in a fermentation medium M
containing tannic acid as the only carbon source. Time
course of enzyme synthesis by the fungus showed that the
enzyme production followed logarithmic growth phase with
maximum enzyme yield being obtained after 6 days
corresponding to the culture pH of 3.8.
Key words:
Tannin, tannase, tannic acid, methyl gallate, Aspergillus
tamari IMI388810 (B), Oji River L.G.A., Enugu, Nigeria. |
