Full Length Research Paper

Potential probiotic of Lactobacillus johnsonii LT171 for chicken nutrition

Hamidreza Taheri¹*, Fatemeh Tabandeh², Hossein Moravej¹, Mojtaba Zaghari¹, Mahmood Shivazad¹ and Parvin Shariati²

¹Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, 31587-11167, Karaj, Iran.

²Industrial and Environmental Biotechnology Department, National Institute of Genetic Engineering and Biotechnology (NIGEB), 14965-161, Tehran, Iran.

*Corresponding author. E-mail: taherihr@gmail.com. Tel: +98 261 2248082.

Fax: +98 261 2246752.

Accepted 14 September, 2009

   Abstract

The objective of this research was to investigate the potential probiotic of Lactobacillus johnsonii LT171. It had aggregation (60 min) and antibacterial effects against Salmonella Enteritidis, Salmonella Typhimurium and Escherichia coli O78:K80. It showed amylase and protease activity and high clear zone in culture medium containing calcium phytate; cell surface hydrophobicity, 85.21 ± 7.27%; resistance to acidic condition (pH 3 for 90 min) and bile salts (in culture medium containing 0.075% ox gall). Also it had resistance to nalidixic acid and neomycine.  This research showed appropriate probiotic properties of L. johnsonii LT171 for chicken nutrition. Hence this strain can complement the characteristics of other strains in multistrain probiotics because of its high clear zone in culture medium containing calcium phytate.

Key words: Lactobacillus johnsonii LT171, probiotic, chicken.