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Full Length Research Paper
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Optimal control of a batch
bioreactor for the production of a novel antifungal
substance CF66I
Chun-Shan Quan1,2*, Xin Li3,4, Jun-Hua
Wang1,2, Wei Zheng1,2 and Sheng-Di Fan1,2
1Life Science
College, Dalian Nationalities University, Economical and
Technological Development Zone, Dalian, 116600, China.
2Key Laboratory of Biotechnology and Bio-Resources
Utilization, State Ethnic Affairs Commission and Ministry of
Education, Dalian Nationalities University, Dalian 116600,
China.
3Dalian Institute of Chemical Physics, Chinese Academy of
Sciences, 457 Zhong-shan Road, Dalian, 116023, China.
4Graduate University of the Chinese Academy of Sciences,
Beijing, 100049, China.
*Corresponding author. E-mail:
mikyeken@dlnu.edu.cn.
Tel.: +86-411-8753-2756 or +86-(0)411-87656005. Fax:
+86-411-87644496.
Accepted 21 July, 2009 |
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Abstract |
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More
than 80% of the plant diseases are caused by fungi. Usually,
fungi not only destroy the plants, but also produce
mycotoxins that are harmful to human health. At present,
chemical fungicides are mainly used for the prevention of
fungi-related plant diseases, however, research and
development of biological prevention and controlling are of
great importance. In this work, the effects of pH and
temperature on cell growth and CF66I formation in batch
culture of Burkholderia cepecia CF-66 were studied.
The
pH value has a marked effect on cell growth and production
of CF66I. The lag phase was much longer when pH set lower
(e.g.5.0) or higher (e.g.8.0). For earlier phase, optimal pH
value was 6.0, because the lag phase can be shortened and
the whole fermentation phase can also be shorten and then
quickly goes into CF66I production phase. In the late phase,
the higher pH is in favor of the production of CF66I.
Different temperature have different effect on cell yield,
specific growth rate, CF66I yield and specific synthesis
rate. In the prophase of fermentation, it is better to set
higher temperature to make the cell growth maximizing as
soon as possible. However in mid-anaphase, lower temperature
shortens the fermentation time, reduce heating energy and
the cost. According about results, an optimal control
strategy was constructed.
Key
words:
Burkholderia cepacia, antifungal activity, optimal
control, CF66I. |
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