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A comparison of efficacy
between conventional and modified methods of the chronic
myocardial ischemia/reperfusion model
Hai-Tao Liu1,
Fei Li1, Yue-Min Wang2, Wayne Bond Lau3,
Ling Tao1, Yuan Yuan1 and Hai-Chang
Wang1*
1Department
of Cardiology, Xijing Hospital, Fourth Military Medical
University, Xi’an, China.
2Department
of Physiology, Fourth Military Medical University, Xi’an,
China.
3Department
of Emergency Medicine, Thomas Jefferson University,
Philadelphia, USA.
*Corresponding author. E-mail:
wanghc@fmmu.edu.cn.
Tel.: 86(29)84775183.
Fax: 86(29)84771170.
Accepted
17 August, 2009 |
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The
objective of this study is to develop and compare the
efficacy of a modified versus conventional rat model of
chronic myocardial ischemia/reperfusion. Sixty Sprague
Dawley (SD) rats were randomly divided into two groups, a
modified group (mask respiratory support and short-time
chest-opening) and a conventional group (tracheal intubation
and long-time chest-opening). Operation time, surgical
success rate, survival rate and infarct size were
investigated. In addition, the post-operative living state
of the rats was observed. In the perioperative period, the
surgical success rate was greater in the modified model (P <
0.05 vs. conventional model). Both chest-opening time and
spontaneous respiration recovery time were significantly
shorter in the modified group versus the conventional model
(P < 0.001). Postoperative resumption of normal behaviors
and activities was quicker in the modified surgical group,
which demonstrated a statistically significant mortality
benefit compared to the conventional group (P < 0.001).
Infarct size, assessed via triphenyltetrazolium chloride
staining, was without statistical difference between the 2
groups (P > 0.05). The modified method offers advantages of
simplicity, efficiency and independent conduct. Its
employment enhances the success rate of the chronic rat
myocardial ischemia/reperfusion model.
Key
words:
Rat, chronic, ischemia/reperfusion model, modified. |
