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Analyses of karyotypes and
comparative physical locations of the resistance gene,
Xa-5, between Oryza sativa and Oryza
officinalis
Liu Hong#, Chen
Yan#, Li Gang, Liu Xue-qun and Qin Rui*
Key
Laboratory of State Ethnic Affairs Commission for Biological
Technology, College of Life Sciences, South-Central
University for Nationalities, Wuhan, 430074, P.R. China.
*Corresponding author. E-mail:
qin_rui@hotmail.com.
Tel: +86 27 6112 4713.
Fax: +86 27 6784 2689.
Accepted 24 September, 2009 |
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A rice BAC
library is being used widely in rice genome researches due
to its distinctive advantages over other library systems.
Physical locations of the rice bacterial blight resistance
gene, Xa-5, was mapped comparatively with a BAC clone
linked to this gene, in Oryza sativa, a
cultivated rice and Oryza officinalis, a wild
rice. Xa-5 was in situ hybridized to O.
sativa and O. officinalis chromosomes. Xa-5
was located on the short arm of chromosome 5 in O. sativa
but on the long arm of chromosome 5 in O. officinalis
with fraction length (FL) 48.85 and 47.30%, respectively and
their FL were consistent with the results obtained by using
the selective marker of rice, RG556, as a probe. The
frequencies of signal detection of the marker, RG556 and the
BAC clone, 44B4, were 8.0 and 41.3% in O. sativa,
while 9.0 and 42.3% in O. officinalis,
respectively. Based on a comparative RFLP map of a wild
rice, O. officinalis and O. sativa,
comparative analyses of karyotypes of O.
officinalis were demonstrated firstly by fluorescent
in situ hybridization (FISH) using a BAC clone and an
RFLP marker from O. sativa as probes.
Key
words:
BAC-FISH, karyotype, Oryza sativa, Oryza
officinalis, physical location, Xa-5. |
