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Altered
membrane permeability in multidrug resistant
Escherichia
coli isolated from extra-intestinal infections
Bindu Arora* and Trupti
Jagdale
Department of Microbiology, Abeda Inamdar Senior
College, Azam Campus, Camp, Pune- 411 001, India.
*Corresponding author. E-mail:
bindu_arora@yahoo.co.uk. Tel: +919766317202.
Accepted
29 September, 2009 |
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The
study was conducted with the objective of examining the
outer membrane proteins and their involvement during the
transport of β - lactams in multidrug resistant
Escherichia coli
isolated from extra-intestinal infections. Also, the
response of gram negative bacterial biomembrane alteration
was studied using extended spectrum β-Lactamase (ESBL)
positive strains of E.coli in the presence and
absence of stress. Outer membrane protein analysis revealed
that multidrug resistance was mediated by loss of porins in
the ESBL isolates of E. coli. Parallel to this, the
level of outer membrane proteins decreased dramatically with
increased antibiotic stress in the isolates. In non-ESBL
isolates of E. coli, porins seems to have no
influence in co-modulation of resistance.
Key
words:
Outer membrane proteins, porins, Escherichia coli,
multidrug resistance,
extra-intestinal, extended spectrum β-Lactamase (ESBL). |
