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RNA recognition motif (RRM)-containing
proteins in Bombyx mori
Lin-Ling Wang and Ze-Yang
Zhou*
College of Life Sciences,
Chongqing
Normal University, Chongqing 400047, China.
*Corresponding author.
E-mail: zyzhou@cqnu.edu.cn. Tel.:
+86-23- 65910315; Fax:
+86-23- 65910312.
Accepted
28 January, 2009 |
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RNA recognition motif (RRM)-containing proteins play
important roles in the processing of RNA and regulation of
protein synthesis. Bombyx mori is a model species of
Lepidoptera whose genome sequence is available; however, its
RRM-containing proteins have not been thoroughly analyzed.
In this study, 134 RRM-containing protein genes in
Drosophila melanogaster were used to perform BLAST search
against B. mori genome- and EST databases. Results
showed that 123 genes have orthologues in the B. mori
genome database and 89 in B. mori EST database,
indicating some of the genes were not expressed in B.
mori. 23 RRM-containing protein genes that have complete
open reading frame were obtained through contig assembly in
B. mori EST database and then registered in NCBI.
Cluster analysis showed that these 23 new proteins formed
two distinct clades. Especially, TAR DNA-binding
protein-43(TBPH) gene of B. mori is different from
that of other species in that it does not have introns.
Moreover, the length of TBPH of B. mori is much
shorter than that of other species’ TBPH, suggesting that it
may play some roles in emergency responses.
Key
words:
Bombyx mori,
RRM-containing protein, TAR
DNA-binding protein-43. |
