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Isolation of gallic
acid-producing microorganisms and their use in the
production of gallic acid from gall nuts and sumac
Nalan Yılmaz Sarıözlü* and
Merih Kıvanç
Anadolu University, Faculty of Science,
Department of Biology, 26470 Eskişehir, Turkey.
*Corresponding author. E-mail: nalany@anadolu.edu.tr.
Tel.: 90 222 3350580 4707; Fax: 90 222 3204910.
Accepted
3 January, 2009 |
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A total number of eighty gallic acid producing strains were
isolated from forest soil or plant samples. Among these
strains, thirteen isolates were selected for gallic acid
production and these isolates were Aspergillus niger
1, A. niger 2, A. niger 3, Penicillium
canescens (3), P. frequentans (2), P.
spinulosum (2), P. purpurogenum (2), and P.
zacinthae. By using eight of these strains and reference
strain of A. niger NRRL 321, the production of gallic
acid from oak tree (Quercus infectoria) gall nuts or
sumac (Rhus coriaria) leaves were investigated.
Maximum gallic acid yields from gall nuts were obtained
for A. niger 3 (91.3%) and P. spinulosum
(93.2%). In the case of sumac leaves, the reference strain
A. niger NRRL 321 (46.1%) and P. zacinthae
(48.2%) gave the highest gallic acid yields. To date, this
study is the first report on production of gallic acid by
these newly isolated Penicillium strains.
Particularly, A. niger 3, P. spinulosum, P.
purpurogenum and P. canescens may be used not
only for gallic acid but also tannase production from tannin
rich plant materials such as gall nuts. Their high yields
and short incubation periods are also remarkable.
Key
words:
Gallic acid, gall nuts, gallotannin, sumac, tannase. |
