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Full Length Research Paper
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Cytokine gene expression
of peripheral blood lymphocytes stimulated by
lipopolysaccharide
Hassan Hussein Musa1,2,
Yunhua Xu1, Baoying Dai1, Xiaoyan Kang1,
Chunhong Zhu1and Guoqiang Zhu1*
1College
of Veterinary Medicine, Yangzhou University, Yangzhou
225009, China.
2Faculty
of Veterinary Science, University of Nyala, Nyala, 155,
Sudan.
*Corresponding author: E-mail:
yzgqzhu@hotmail.com.
Accepted
23 January, 2009 |
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Abstract |
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Lipopolysaccharide (LPS) is a predominant glycolipid in the outer
membranes of gam-negative bacteria that stimulates monocytes,
macrophages, and neutrophils to produce cytokines. The aim
was to study the expression profile of TLRs and cytokines
and determine the role of LPS in the peripheral blood
lymphocytes. Lymphocytes were isolated from the peripheral
blood of chickens and induced with LPS,
total
RNA was extracted
and reverse
transcribed into cDNA. The mRNA expression level of TLRs and
inflammatory cytokines were quantitatively determined by
semi-quantitative RT-PCR. The result showed that lymphocytes
were expressed mRNA of TLRs (TLR1/6/10, TLR3, TLR4, TLR5 and
TLR7) and interleukins (IL-1β, IL-8,
IL-18 and TGF-β4).
LPS will significantly (P<0.05) induce the expression of
TLR4, IL-1β, IL-8 and IL-18, and non significantly (P>0.05)
induce the mRNA expression of TLR1/6/10, TLR5 and TGF-β4.
LPS plays
an important role in the
innate immune response of lymphocytes
to pathogen.
Key
words:
Lipopolysaccharide,
lymphocytes, TLRs, cytokines. |
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