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Specific and Rapid
Detection of Camellia oleifera Anthracnose Pathogen by
Nested-PCR
Junang Liu1,2,
Li He1,2*, Guoying Zhou1,2
1Biotechnology
Core Facilities, Central South University of Forestry and
Technology, Changsha, 410004, China.
2College
of Forestry, Central South University of Forestry and
Technology, Changsha, 410004, China.
*Corresponding
author. E-mail:
csuftlihe@163.com.
Accepted
3 February, 2009 |
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Camellia
oleifera
is an economical important plant in southern China
for edible oil production. Anthracnose is a serious disease
that limited its development. The internal transcribed
spacer (ITS) regions and the 5.8S rRNA gene of strain C1 of
the pathogenic fungus Colletetrichum gloeosporioides
were sequenced in order to design specific PCR primers for
pathogen detection. Alignment of the sequence data of strain
C1 and the other Colletetrichum species obtained from
the Genbank were made using CLUSTAL W. Based on the aligned
ITS sequences, specific primers for C. gloeosporioides
were developed (YT1 and YT2). The infecting pathogens were
successfully detected with our specific primer set and
showed high specificity. The result showed that the nested-PCR
reaction was at least 10,000-fold more specific than that of
the simple PCR method. This new method provides a useful
technique to further study disease cycle and for early
prediction of anthracnose of Camellia oleifera.
Key words:
Camellia
oleifera,
anthracnose, taxon-specific primer, molecular detection,
nested PCR. |
