Full Length Research Paper

Application of SRAP in the genetic diversity of Pinus koraiensis of different provenances

Fujuan Feng¹, Minmin Chen², Dongdong Zhang¹, Xin Sui¹ and Shijie Han²*

¹Faculty of life science, Northeast Forestry University, Harbin 150040, China.

²Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.

*Corresponding author. E-mail: ffj9018@sina.com. Tel.: +86-13796628587.

Accepted 23 February, 2009

The study applied sequence-related amplified polymorphism (SRAP) technique into genetic diversity of Pinus koraiensis. A total of 480 samples from 24 provenances were amplified by using selected 9 primer pairs. The band number amplified by each primer pair ranged from 24 to 33, with the molecular weight between 100 and 1,500 bp. In total 249 bands were observed, of which 143 were polymorphic (55.42%). No significant difference in the genetic diversity was observed among the provenances, and the maximum and minimum level was found in Dahailin and Bajiazi provenances, respectively. It indicated that no close relationship can be well established between genetic diversity and the location of provenance. The genetic variation mainly caused by the variation of intra population, accounting for 92.35% of the total genetic variation. Moreover, the gene flow of P. koraiensis between the provenances was 2.905, and the relative high gene flow can prevent efficiently gene drift. Based on the UPGMA cluster diagram, the 24 provenances may be divided with the genetic distance of 0.013 into three groups. The result is not completely consistent with the traditional division of provenances.

Key words: Genetic diversity, geographic provenances, Pinus koraiensis, sequence-related amplified polymorphism (SRAP).