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African Journal of Biotechnology

     
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  Afr. J. Biotechnol.

  Vol. 8 No. 6

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  Cebesoy S
 

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African Journal of Biotechnology Vol. 8 (6), pp. 11601164, 20 March 2009

ISSN 1684-5315  © 2009 Academic Journals  

 

 

Full Length Research Paper

 

Morphology and histochemistry of primary flight muscles in Rhinolophus mehelyii

 

Suna Cebesoy

 

Ankara University, Faculty of Science, Deparment of Biology, 06100 Tandogan, Ankara, Turkey. E-mail: suna.cebesoy@gmail.com.

 

Accepted 13 January, 2009

 
   Abstract
 

Two primary flight muscles of Rhinolophus mehelyii were studied using morphological and histochemical analysis. Two fast-twitch fiber types are histochemically identified in pectoralis muscles of R. mehelyii. These were classified as type IIa and type IIb according to glycine-calcium-formalin preincubation staining protocol for myosin ATPase. The primary flight muscles, serratus ventralis included type I, type IIa and type IIb fibers. Type I fibers were highly oxidative, as stained dark for NADH-TR. Type IIa fibers exhibited relatively weak staining properties for NADH-TR and SDH, indicating an intermediate oxidative capacity. Type IIb fibers showed low oxidative capacity, as indicated by weak reaction for NADH-TR.

 

Key words: Rhinolophus mehelyii, skeletal muscles, ATPase, NADH-TR, SDH, histochemical tests.

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