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Genetic variability in
Asparagus racemosus (Willd.) from Madhya Pradesh, India
by random amplified polymorphic DNA
Neetu Vijay1*,
Pramod Sairkar1, Nipun Silawat1, R. K.
Garg1 and N. N. Mehrotra1,2
1Centre
of Excellence in Biotechnology, M. P. Council of Science and
Technology Vigyan Bhawan, Nehru Nagar, Bhopal-462003, Madhya
Pradesh, India.
2Central
Drug Research Institute, Lucknow-226001, India.
*Corresponding author. E-mail:
neetu_2178@rediffmail.com.
Accepted 3
April, 2009 |
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Random amplified polymorphic DNA (RAPD) markers were used to
assess genetic diversity in Asparagus racemosus (Willd.)
an important medicinal plant collected from 7 different
locations covering Madhya Pradesh. High level of genetic
similarity was observed in the collected accessions. 4
random primers generated a total 39 polymorphic bands out of
the total 71 bands. A low genetic diversity among the A.
racemosus samples was revealed by RAPD analysis. Cluster
analysis based on dice coefficient showed 2 major groups.
This distributive pattern of genetic variation of A.
racemosus accessions provides important baseline data
for conservation and collection strategies for this species.
Key
words:
Genetic diversity, Asparagus racemosus, RAPD,
polymorphism. |
