Full Length Research Paper

Characterization of a new synthetic wheat – Aegilops biuncialis partial amphiploid

Feiquan Tan^1,2, Jianping Zhou²*, Zujun Yang², Yong Zhang², Li Pan² and Zhenglong Ren^1,2*

¹State Key Laboratory for Plant Genetics and breeding, Sichuan Agricultural, University, Ya’an, Sichuan, P.R., China.

²Shcool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, P.R., China.

*Corresponding authors. E-mail: zhoujp@uestc.edu.cn. Tel: 86-28-83206556 and renzl@uestc.edu.cn. Tel: 86-835-2882123.

Accepted 17 April, 2009

   Abstract

The aim of the experiments was to identify the synthetic wheat – Aegilops biuncialis germplasm Line 15-3-2 with 42 chromosomes. Morphologically, the spike of line 15-3-2 is intermediate to those of its wheat and Aegilops parents. Line 15-3-2 displays stable fertility and immunity to wheat powdery mildew and stripe rust. The seed storage protein electrophoresis showed that Line 15-3-2 presented almost all gliadin bands and a majority of glutenin subunits of its parents. Fluorescence in situ hybridization (FISH) suggested that Line 15-3-2 contained all 14 D-genome chromosomes and chromosome 5U. Therefore, it could be concluded that Line 15-3-2 is a new synthetic wheat – A. biuncialis partial amphiploid, and could be used to transfer the disease resistance genes to wheat.

Key words: SDS-PAGE, A-PAGE, fluorescence in situ hybridization, wheat – Aegilops biuncialis partial amphiploid.