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The influence of
gibberellic acid and paclobutrazol on induction of somatic
embryogenesis in wild type and hairy root cultures of
Centaurium erythraea Gillib.
Angelina Subotić*, Slađana Jevremović, Milana Trifunović,
Marija Petrić, Snežana Milošević and Dragoljub Grubišić
Department
of Plant Physiology, Institute for Biological Research
“Siniša Stankoviċ”, University of Belgrade, Bulevar despota
Stefana 142, 11060 Belgrade, Serbia.
*Corresponding author. E-mail:
heroina@ibiss.bg.ac.yu. Tel: + 381 11 20 78 425. Fax: +
381 11 27 61 433.
Accepted
2 January, 2009 |
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The effects of exogenous gibberellic acid (GA3)
and paclobutrazol on induction of somatic embryos in wild
type and hairy root culture of Centaurium erythraea
Gillib. were investigated. Both compounds were
incorperated into 1/2 MS medium
at 6 concentrations (0.01, 0.03, 0.1, 0.3, 1.0 and 3.0 µM).
Wild type root and hairy
root explants cultured in the
presence of GA3 at all tested
concentrations under 16-h photoperiod or in the darkness
decreased the number of somatic embryos
that were produced.
Paclobutrazol (0.3
mM)
induced the largest number (19.7, 16.5) of somatic embryos
in wild type and hairy root cultures, respectively. Rooting
of plants derived from
somatic embryos as achieved on ˝MS medium. These results
indicate that paclobutrazol is beneficial for somatic embryo
induction and formation in wild type and hairy root culture.
Key
words:
Hairy root, medicinal plants, root explants, somatic
embryos. |
