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Production and
characterization of endo-b-1,4-glucanase
from thermophilic fungus
Muhammad Asam Raza* and Shafiq-Ur-Rehman
Department
of Chemistry, Government College University, Lahore,
Pakistan, 54000.
*Corresponding author. E-mail:
asamgcu@yahoo.com.
Accepted 21
February, 2008 |
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Chaetomium thermophile
produces substantial extra-cellular cellulase on several
cellulosic substrates (wheat straw, wheat bran and corn
straw etc.) including simple sugars. 2% (w/v) wheat straw,
wheat bran and corn straw were used as carbon source and
urea was used as a nitrogen source.
Experiment was
conducted in a 20-l stirred tank reactor under the following
conditions: pH 5.0, stirring speed 300 rpm, temperature 45oC
and aeration 50% (DO).
The samples were
withdrawn
after every 12 h regularly and enzyme activity
was assayed. Endo-b-1,4-glucanase
activities on wheat straw, wheat bran and corn straw were
2.51, 1.62 and 5.10 U/ml, respectively and extra-cellular
protein contents were 1.48, 1.17 and 2.88 mg/ml,
respectively.
Specific activity of enzyme
after ammonium sulphate precipitation and
Fast
Protein Liquid Chromatography (FPLC) was
2.1 and 3.3 U/mg of protein, respectively.
SDS-PAGE showed only a single band at 53 kDa. The optimum
temperature and pH of the CMCase were 60oC and
from 6.0 - 7.5, respectively.
Key words:
Chaetomium thermophile,
cellulase, fermentation, extra-cellular enzymes, agro-wastes. |
