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Influence of explanting
season on in vitro multiplication of the medicinal
herb, Tridax procumbens L.
C. P. Malik* and Chitra
Wadhwani
School of
Life Sciences, Jaipur National University, Jaipur, India.
*Corresponding author.
E-mail:
cpm_malik@yahoo.com.
Accepted 21
May, 2009 |
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A protocol for complete regeneration of Tridax procumbens,
a medicinally important plant, was accomplished through
in vitro culturing. From nodal segments explants, high
percentage of bud break and multiple shoot formation was
induced between July and September on MS medium supplemented
with BAP (1 mg L-1). Rooting of the excised
shoots from secondary cultures was excellent on
half-strength MS medium having 1 mg L-1
IBA. Micropropagated plants when transplanted on garden soil
flowered after 6 - 8 weeks. Explants sampled during December
had low percentage of bud break and less shoot number per
explant. Epicuticular wax (EW) and cuticular transpiration
varied in the leaves collected during July and December;
while under in vitro conditions, no marked
differences were observed in regards to the 2 parameters.
The epicuticular contents correlated with the rate of
cuticular transpiration. The adaptive significance of EW
during December is discussed.
Key
words:
Tridax procumbens, medicinal plant, micro
propagation, explanting season, epicuticular wax. |
