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Genomic growth hormone,
growth hormone receptor and transforming growth factor
b-3 gene polymorphism
in breeder hens of Mazandaran native fowls
Babak Enayati and Ghodrat
Rahimi-Mianji*
Laboratory
for Molecular Genetics and Animal Biotechnology, Department
of Animal Sciences, Faculty of Animal Sciences and Fishery,
Sari Agricultural Sciences and Natural Resources University,
Sari, Iran.
*Corresponding author. E-mail:
rahimimianji@yahoo.com. Tel.: +98-911-1513087. Fax:
+98-151-3822577.
Accepted
19 March, 2009 |
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Growth hormone axis and the transforming growth factor-b
subfamily are the most important groups of genes that are
involved in a wide variety of physiological
functions such as growth and reproduction. As no information
about the allelic characterization of growth hormone (GH),
growth hormone receptor (GHR) and transforming growth factor
b3
(TGF-b3)
loci is available in breeder hens for Mazandaran native
fowls breeding station, we studied their distribution in
this population. A total of 156 blood samples were collected
and a specific primer sets were used to amplify a fragment
of GH, GHR and TGF-b3 loci using polymerase chain reaction (PCR). The PCR
products from GH, GHR and TGF-b3
loci were digested with SacI, HindIII and
BslI restriction endonuclease, respectively. In GH and
GHR loci, allele A was the most frequent and ranged from
0.99 to 0.79 while, allele B was identified as a dominant
allele at TGF-b3
locus due to the highest frequency (0.81). The frequency of
BB homozygous genotype was the lowest (average = 0.03)
whereas, AA genotype showed the highest frequency among all
loci. The amplified fragment in GH locus was characterized
by a deletion of approximately 118 bp. Deletion of 118 bp
fragment not only reduced the expected size of
the PCR product, but also, introduced a new restriction site
for SacI enzyme at GH marker site. Further
association analysis is required to clarify the effects of
these marker genotypes on production traits in this breeder
flock.
Key
words:
GH, GHR, TGF-b3,
breeder hen. |
