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RAPD-based detection of
genomic instability in cucumber plants derived from somatic
embryogenesis
Khaled M. Suliman Elmeer1*,
Thomas F. Gallagher2 and Michael J. Hennerty2
1Plant
Tissue Culture Laboratory. Department of Water Research and
Agriculture Doha-Qatar.
2School
of biology and Environmental Sciences, Belfield, Dublin 4,
Ireland.
*Corresponding author.
E-mail: elmeer@gmail.com.
Accepted 8
May, 2009 |
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Random amplified polymorphic DNA (RAPD) markers were used to
evaluate genetic stability of regenerants of cucumber plants
obtained through somatic embryogenesis.
Somatic embryo plants and plants of F1 hybrids, from which they were
derived, were compared during weaning, early growth,
flowering, fruiting and at maturity. No differences in
phenotype were observed, by simple observation.
Banding patterns were scored for the presence (+) or absence
(-) of a DNA band, there were no visually detectable
differences between the somatic embryo derived plants
compared to their F1 parents in the Random Amplified
Polymorphic DNA (RAPD) test using five primers OP-C10,
OP-G14,
OP-H05,
OP-Y03 and
OP-AT01.
The results indicate the usefulness of RAPD markers to
detect genetic instability in cucumber primary regenerant
plants derived from somatic embryogenesis, and as a
certification tool for monitoring genetic stability during
the generation process.
Key
words:
Somaclonal variation, genetic instability. |
