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Lectin receptor kinase
LecRK-b2 localizes to plasma membrane and functions as a
homdimer
Keqin Deng, Xiushan Li, Dashi Yu, Qiming Wang, Jianxin
Zeng, Xiaoying Zhao, Dongying Tang and Xuanming Liu*
Bioenergy
and Biomaterial Research Center, Bioscience and
Biotechnology Institute, Hunan University, Changsha 410082,
China.
*Corresponding author. E-mail:
sw_xml@hnu.cn. Fax:
+86-0731-8821721.
Abbreviations: LecRK, Lectin receptor kinase; RLK, receptor
like kinase; GFP, green fluorescent protein; LUC, luciferase;
EDTA, ethylenediaminetetraacetic acid.
Accepted 10
July, 2009 |
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An Arabidopsis putative lectin receptor-like serine/threonine
kinase gene, LecRK-b2, has been characterized. Confocal
microscopy images showed that the LecRK-b2-GFP fusion
protein is localized to plasma membrane. The results of
yeast 2 hybrid showed that lectin domain of LecRK-b2 had
self-interaction, while the kinase domain did not work.
Split luciferase complementation assays confirmed the yeast
two hybrid results and suggested that lectin domain was
responsible for the dimerization of LecRK-b2 protein in
plant. Furthermore, the recombinant LecRK-b2 protein
exhibited autophosphorylation activity.
Key
words:
Lectin receptor kinases, subcellular localization,
homodimer. |
