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A comparative study of
Thermomyces lanuginosus strains on thermostable xylanase
production
Khwanchai Khucharoenphaisan1, Shinji Tokuyama2,
Khanok Ratanakhanokchai3 and Vichein
Kitpreechavanich1*
1Department
of Microbiology, Faculty of Science, Kasetsart University,
Bangkok 10900, Thailand.
2Department
of Applied Biological Chemistry, Faculty of Agriculture,
Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan.
3School
of Bioresources and Technology, King Mongkut’s University of
Technology, Thonburi, Bangkok 10140, Thailand.
*Corresponding author. E-mail:
fsciwck@ku.ac.th.
Tel.:+81-2-5792351. Fax: +81-2-5792081.
Accepted
2 March, 2009 |
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Strains of Thermomyces lanuginosus could be
differentiated into two groups based on their ability to
produce xylanase using xylan or xylose as sole of carbon
source. One group of these strains produced high xylanase
activity either in the medium using xylan or xylose as a
sole of carbon source. The xylanase production by T.
lanuginosus ATCC 44008, THKU-11, and THKU-25, which were
the representative members of this group, increased when
xylose was added to the xylan medium. In contrast, there was
another group that produced high xylanase activity only in
the xylan medium. Addition of xylose to the xylan medium
resulted decreasing of xylanase production in T.
lanuginosus ATCC 46882, TISTR 3465 and THKU-85 that
belonged to this group. The finding indicated that induction
and repression mechanism of xylose on xylanase expression
was different among the strains of T. lanuginosus. In
addition, phylogenetic analysis obtained from random
amplified polymorphic DNA (RAPD) pattern using primer UBC
241 pointed to greater diversity of high and low xylanase
producing strains using xylose as a carbon source.
Key
words:
Thermomyces
lanuginosus, RAPD, thermostable xylanase |
