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Determination of bacterial and fungal numbers in floats of
pre-tanning operations
S. T. Bilgi1, B. Mericli
Yapici1* and A. N. Yapici2
1Department
of Biology, Faculty of Science and Arts, University of
Canakkale Onsekiz Mart, 17020 Canakkale, Turkey.
2Biga
Vocational College, University of Canakkale Onsekiz Mart,
17200 Biga, Canakkale, Turkey.
*Corresponding author. E-mail:
yapici69@gmail.com.
Tel.: +(90)2862180018. Fax: +(90)2862180533.
Accepted 2 March, 2009 |
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This study was aimed at determining the numbers of bacteria
and fungi in the floats of main soaking, liming, deliming-bating,
degreasing and pickling stages. To this end, the numbers of
total aerobic mesophilic, proteolytic, lipolytic and aerobic
spore-forming bacteria and, the numbers of total aerobic
fungi (yeast and mould), proteolytic and lipolytic fungi
were determined on the media containing different
concentration of NaCl (0, 5, 10, 15 and 25%). Sheep skins
were processed up to the end of pickling stage by adding a
commercial bactericide (Derbio® DB 99), whose
active ingredient is composed of quarternized compounds,
into main soaking float. According to the results of the
study, while most of the bacteria displayed growth from the
main soaking process to the end of deliming-bating, most of
the fungi showed growth from the main soaking process to
degreasing. It is found that main soaking and deliming-bating
processes came to the fore in terms of bacterial growth. It
is also remarkable that the numbers of proteolytic bacteria
were higher in degreasing float as well as in main soaking
float. In the study, fungal numbers were found to be lower
in all the stages than bacterial numbers in general.
Moreover, fungi displayed growth in all NaCl concentrations
(0, 5, 10 and 15%) in all the processes up to the end of the
degreasing process except on the media containing 25% NaCl
for the main soaking float. As for in pickling float, only
proteolytic fungi displayed growth on the media containing 0
and 5% NaCl.
Key words:
Beamhouse operations, proteolytic bacteria, lipolytic
bacteria, fungi, leather industry. |
