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Study on genetic
diversity in Pakistani wheat varieties using simple sequence
repeat (SSR) markers
Bahadar Zeb, Imtiaz Ahmad Khan, Shahid
Ali*, Sardar Bacha, Saqib Mumtaz and Zahoor Ahmed Swati
Institute of Biotechnology and Genetic Engineering NWFP,
Agricultural University, Peshawar-Pakistan.
*Corresponding author.
E-mail:
shahid2_khan@hotmail.com. Tel.: +92-91-9216553, Fax:
+91-91-9218102.
Accepted 22 June, 2007 |
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Common wheat (Triticum aestivum L.) is a grass
species, cultivated world wide. Globally, it is the most
important human food grain and ranks second in total
production as a cereal crop behind maize. Genetic diversity
evaluation of germplasm is the basis of improvement in wheat.
In the present study genetic diversity of 10 varieties of
wheat (T. aestivum) were analyzed using 14 simple
sequence repeat (SSR) primer sets. To estimate the genetic
diversity among the genotypes, bivariate data matrix was
generated and genetic distances were calculated using
unweighted pair group of arithmetic mean (UPGMA) procedure.
A high degree of genetic polymorphism was observed among the
wheat varieties with average genetic distances ranged from
16 to 67%. SSR primer gdm-3, gdm-19, gdm-61, gdm-62, gdm-64,
gdm-86, gdm-88, gdm 93-2a, 93-4b, gdm-13 and gdm-115
amplified 3, 2.9, 4.1, 4.7, 3, 1.7, 2.7, 3.7, 5.8, 4.1 and
1.4 loci per variety, respectively. Most diverse varieties
of wheat were identified (Punjab-81 and Indus-79) and
recommendations were made to utilize these varieties in
future breeding program.
Key words:
Triticum aestivum
L., SSR markers, genetic diversity. |
