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Full Length Research Paper
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Exogenous
cycilc AMP and cycilc GMP influence the metabolism of traces
of tritium-labeled glycerol in rabbits
Yongfeng Liu,
Linsen Zan*, Fang Wei, Dengke Liu, Yaping Xin and Wanqiang
Tian
College of Animal Science and Technology, Northwest A & F
University, Yangling, Shaanxi, 712100, Republic of China.
*Corresponding author. E-mail:
zanls@yahoo.com.cn.
Tel.: +86-29-87091923.
Fax: +86-29-87091148.
Accepted 13 July, 2009 |
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Abstract |
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To characterize the dynamic distribution and metabolism of
endogenous glycerol in vivo, the exogenous glycerol
labeled by radioactivity was mimicked to trace its kinetics
in Chinese Haerbin white rabbits. Based on the kinetic
alternations, the metabolic equation of 3H-glycerol
was formulated. The determining coefficient (R2)
was 0.9876 closed to predicted value (p < 0.05). The
proposed equation fits well with the metabolic dynamics of
exogenous 3H-glycerol within 30 h after
injection. Moreover, according to the equation, the
exogenous 3H-glycerol accumulates mainly in
kidney, intestine and fat after 95 h injection. Also the
dynamic concentration of 3H-glycerol in the
examined kidney to intestine to fat and other tissues
decreases gradually, indicating a underlying kinetic feature
in Chinese Haerbin white rabbits. In addition, the applied
exogenous
cAMP and cGMP
show a distinct impact on glycerol distribution.
Key words:
3H-Glycerol, Haerbin white rabbits, trace
kinetics, cAMP, cGMP. |
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