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Full Length Research Paper
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RAPD analysis for genetic diversity of two populations of
Mystus vittatus (Bloch) of Madhya Pradesh, India
R. K. Garg1*, N. Silawat1, Pramod
Sairkar1, Neetu Vijay1 and N. N.
Mehrotra2
1Centre
of Excellence in Biotechnology, M.P. Council of Science and
Technology, (MPCST) Vigyan Bhawan, Nehru Nagar,
Bhopal-462003 (M.P.), India.
2Central
Drug Research Institute (CDRI), Lucknow-226001 (U.P.),
India.
*Corresponding author. E-mail:
gargrajk@rediffmail.com or
rkgargmpcst@gmail.com
Tel.: 0755-2670447. Mobile: 09826220136.
Accepted 20 July, 2009 |
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Abstract |
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The random
amplified polymorphic DNA polymerase chain reaction (RAPD-PCR)
was applied to analyze the genetic variation of the 2
populations of Mystus vittatus (Bloch) of Madhya
Pradesh, India. 10 random 10-mer primers were primarily
scored in 3 individuals from each of the 2 locations. Five
primers, which gave polymorphism, were selected for
polymerase chain reaction (PCR) and used in the final RAPD
analysis of 20 individuals collected from 2 sites, namely
Bhadwada reservoir (Bhopal) and Mohinisagar reservoir
(Gwalior). These primers produced 388 scorable DNA fragments
were found, of which 252 (64.98%) were polymorphic, 182
(46.90%) were monomorphic and 14 (3.61%) were unique. RAPD
banding patterns, showed variations between and within the
populations, while, the morphological variations were
negligible.
Key
words:
Catfish, genetic diversity, RAPD, polymorphism. |
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