Full Length Research Paper

Molecular cloning and characterization of a new peroxidase gene (OvRCI) from Orychophragmus violaceus

Dai Qilin^1,2, Wang Jin^2,3, Feng Bin³, Liu Tingting³, Chen Chen⁴, Lin Honghui¹ and Du Shizhang²*

¹Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu 610064, China.

²Plant Genomics Research center, Mianyang Normal College, Mianyang 621000, China.

³Plant Biotechnology Research Centre, Southwest University of Science and Technology, Mianyang 621000, China.

⁴School of Pharmacy, Fudan University, Shanghai 200030, China.

*Corresponding author. E-mail: wjdsz@vip.sina.com.cn or honghuilin@vip.sina.com.

Accepted 5 November, 2009

   Abstract

A new peroxidase gene from Orychophragmus violaceus was cloned. The full-length cDNA of O. violaceus peroxidase gene (OvRCI, GenBank. Acc. No. AY428037) was 1220 bp and contained an 1128 bp open reading frame encoding a protein of 375 amino acids. Homology analysis and molecular modeling revealed that OvRCI strongly resembled other peroxidase genes. Quantitative real-time PCR analysis revealed that it was a constitutively salt-inducible gene and its transcript level was most abundant after 24 h treatment with 200 mmol.L^-1 sodium chloride. Our studies suggested that OvRCI was a new member of the family of recently cloned peroxidase genes.

Key words: Orychophragmus violaceus, OvRCI, quantitative real-time PCR.