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The consequences of the
effects of the chemotherapeutic drug (vincristine) in organs
and the influence on the bioavailability of two radio-biocomplexes
used for bone evaluations in balb/c female mice
Deise Mara Machado de Mattos1,2,7*, Diré, G.
F.1,2,4,5, Lima, R. C.1, Almeida, A.
C. C.1, Bellucio D.4, Azevedo, C. S.
S.1, Azevedo, S. S. S.1, Nascimento,
S. F.1, Borba, H. R.4, Carvalho, J. J.5
and Bernardo Filho, M.2,6
1Universidade
Estácio de Sá. Centro de Ciências da Saúde. Rio de Janeiro,
RJ, Brazil.
2Universidade
do Estado do Rio de Janeiro, Instituto de Biologia Roberto
Alcantara Gomes, Departamento de Biofísica e Biometria, Rio
de Janeiro, RJ, Brazil.
3Universidade
do Estado do Rio de Janeiro, Instituto de Biologia Roberto
Alcantara Gomes, Departamento de Histologia, Rio de
Janeiro, RJ, Brazil.
4Universidade
Federal Rural do Rio de Janeiro, Instituto de Biologia,
Departamento de Biologia Animal, Laboratório de Atividade
Anti-helmíntica de Plantas. Seropédica, Rio de Janeiro, RJ,
Brazil.
5Centro
Universitário da Zona Oeste- UEZO,
Avenida Manuel Caldeira de Alvarenga, 1203. Campo Grande, RJ
23070-200, Brazil.
6Instituto
Nacional do Câncer, Coordenadoria de Pesquisa, Praça Cruz
Vermelha, Rio de Janeiro, RJ, Brazil.
7Universidade
Estácio de Sá, Instituto das Ciências da Saúde, Centro de
Pesquisas da Saúde - Campus R9, Rua André Rocha, 838,
Taquara - Jacarepaguá, Rio de Janeiro, RJ, Brasil,
22740-361.
*Corresponding author. E-mail:
deisemar@hotmail.com.
Tel: + 552133126123. Fax: + 552133126100.
Accepted 16
october, 2008 |
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The development of animal model to evaluate the toxicological
action of compounds used as pharmaceutical drugs is desired.
The model described in this work is based on the capability
of drugs to alter the bioavailability of
radiopharmaceuticals (radiobiocomplexes) labeled with
technetium-99 m (99mTc). There are evidences that
the bioavailability or the pharmacokinetic of
radiobiocomplexes can be modified by some factors, as drugs,
due to their toxicological action in specific organs.
Vincristine is a natural product that has been utilized in
oncology. The vincristine effect on the bioavailability of
the radiobiocomplexes 99mTc-methylenediphosphonic
acid (99mTc-MDP) and 99mTc-pyrophosphate
(99mTc-PYP) in Balb/c female mice was evaluated.
The fragments of kidney were processed to light microscopy
and transmission electron microscopy. The aim of this work
was to study at structural and ultrastructural levels
the alterations caused by vincristine in organs. One
hour after the last dose of vincristine, 99mTc-PYP
or 99mTc-MDP
was injected, the animals were sacrificed and the percentage
of radioactivity (%ATI) was determined in the isolated
organs. Concerning 99mTc-PYP, the %ATI (i)
decreased in spleen, thymus, lymph nodes (inguinal and
mesentheric), kidney, lung, liver, pancreas, stomach, heart
and brain and (ii) increased in bone and thyroid.
Concerning 99mTc-MDP,
the %ATI (iii) decreased in
spleen, thymus, lymph nodes (inguinal and mesentheric),
kidney, liver, pancreas, stomach, heart, brain, bone, ovary
and uterus. In conclusion, the toxic effect of vincristine
in determined organs could be responsible for the alteration
of the uptake of the studied radiobiocomplexes.
Key
words:
Radiobiocomplexes, vincristine, drug interaction, nuclear
medicine, oncology. |
