Full Length Research Paper

Antimicrobial and cytotoxic activity of Streptomyces sp. from Lonar Lake

Kiran R. Kharat¹* Amol Kharat² and B. P. Hardikar²

¹Department of Biotechnology, Deogiri College, Aurangabad, 431001, India.

²Rofel College of Pharmacy, Vapi, Gujarat, KET’s V.G.Vaze College, Mulund, Mumbai.

*Corresponding author. E mail: biotech@deogiricollege.org. Tel: 0091 240 2334577, extn. 27. Fax: 0091 240 2334430.

Accepted 23 September, 2008

   Abstract

Antibacterial substances from actinomycetes were isolated from marine environment of Lonar Lake and characterized. Out of the 24 isolates subjected to secondary screening, 12 isolates were active against Bacillus subtilis, 13 against Staphylococcus aureus, 7 against Escherichia coli, 3 against Proteus vulgaris  and 4 against Salmonella typhi. Metabolites in the extract of broth of 48 hrgrown Streptomyces spp. culture no.2 proved to have antimicrobial and cytotoxic against human lung carcinoma cell A549.

Key words: Actinomycetes, antimicrobial activity, cytotoxic activity, human lung carcinoma cells -A549.