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Water stress induced
changes in antioxidant enzymes, membrane stability and seed
protein profile of different wheat accessions
Sumera Iqbal and Asghari
Bano*
Department
of Plant Sciences, Quaid-i-Azam University, Islamabad.
*Corresponding author. E-mail:
asgharibano@yahoo.com.
Accepted
3 November, 2009 |
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Water
stress induced changes in antioxidant enzymes membrane
stablity index and seed protein profiling of four different
wheat (Triticum aestivum L.) accessions (011251,
011417, 011320 and 011393) were determined in a pot study
under natural condition during the wheat-growing season 2005
and 2006. Sampling was done 3, 6 and 9 days after induction
of water stress. Recovery was studied at 48 and 72 h of
re-watering. Marked increase in leaf antioxidant enzymes
associated with a decrease in membrane stability index
occurred under water stress. Accession 320 showed the least
increase in catalase and peroxidase activity but maximum
decrease in membrane stability index. The inhibitory effects
of water stress on plants were ameliorated by exogenous
application of ABA and this ameliorating effect was found to
be more significant at booting stage as compared to
grainfilling particularly in the accession 320. The
accessions 417 and 320 (which were most dissimilar on the
basis of physiology under water stress, one most tolerant
and the other most sensitive to water stress) showed least
polymorphism among the four accessions on the basis of RAPD
(Random Amplified Polymorphic DNA) analysis. Seed protein
composition was found to be mainly controlled by genetic
factors rather than water stress.
Key
words:
Grainfilling, booting, protein, antioxidants, wheat. |
