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Effects of
organic nitrogen and carbon
sources on mycelial growth and polysaccharides production
and their optimization in the
submerged culture of
Grifola umbellate, a Chinese medicinal herb
Yuan Zhou1,
Shao Hong-bo1,2,3,4* and De-yu Chen1
1Institute
of Soil and Water Conservation, Chinese Academy of Science
and Ministry of Water Resources, Northwest A&F University,
Yangling 712100, China.
2Shandong
Provincial Key Laboratory of Eco-environmental Science for
Yellow River Delta, Binzhou University, Binzhou
256603,China..
3Yantai
Institute of Costal Zone Research, Chinese Academy of
Sciences (CAS), Yantai264003, China.
4Institute
for Life Sciences, Qingdao University of Science and
Technology (QUST), Qingdao 266042, China.
*Corresponding author. E-mail:
shaohongbochu@126.com.
Tel.:+86 53284023984.
Accepted
24 August, 2009 |
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Grifola
umbellate
is a famous and expensive Chinese herb medicine and the main
medicinal component is
polysaccharide mainly produced by its mycelia. Effects of
organic nitrogen and carbon resources on mycelial growth and
polysaccharides production of a medicinal mushroom, G.
umbellate were studied in the submerged culture system.
Soybean meal was selected as the optimal organic nitrogen
source for its significant promotion effects on mycelial
growth and polysaccharides synthesis based on results of an
orthogonal matrix experiment design. Glucose was proved to
be a preferred carbon source by comparison with fructose and
sucrose. The optimal dosages of soybean meal 5.63% (w/v) and
glucose 4.33% (w/v) were obtained, respectively through the
curvilinear regressions experiment. The productions of
mycelial dry weight, exo-polysaccharide (EPS) and inner
polysaccharide (IPS), in the optimal medium, were about
3.16, 1.48 and 2.78 folds higher than those in the basal
medium, respectively and culture duration was shortened to
half through optimizing the organic nitrogen and carbon
sources. These results
will be
significant contribution to process biochemistry of
medicinal fungus in the submerged culture system.
Key
words:
Grifola umbellate,
Chinese medicinal herb.
polysaccharides, submerged culture, organic nitrogen source,
carbon source,
orthogonal design. |
