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pLIVE-EGFP: A liver
specific vector carrying the EGFP reporter for transgenic
screening and expression
Suhua Zhang1#,
Xiaojun Ma1#,
Jinbao Tang2*
and Shujuan Liang1*
1Department
of Immunology, Key laboratory for immunology in universities
of Shandong Province, Weifang Medical University, WeiFang,
Shandong Province, 261053, People’s Republic of China.
2Department
of Basic Medicine, Weifang Medical University,
7166
Baotong Xijie,
WeiFang, Shandong Province, 261053, People’s Republic of
China.
*Corresponding authors. E-mail:
liangshjuan@yahoo.cn
(S.J. Liang),
tangjb@wfmc.edu.cn (J.B. Tang).
#These
authors contributed equally to this paper
Accepted
10 August, 2009 |
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pLIVE-EGFP is a liver-specific expression vector constructed
by insertion of IRES-EGFP gene at the Xho I site of
the pLIVE vector. The pLIVE-EGFP vector permits simultaneous
expression of a gene of interest in addition to the EGFP
reporter, specifically within liver cells, both in vivo
and in vitro. When expressed in liver cells, the
EGFP reporter produces a bright green fluorescence, which
can easily be detected with fluorescence microscopy or flow
cytometry. Compared with its parental vector, the pLIVE-EGFP
is more powerful in providing a simple, direct method for
monitoring gene transfection efficiency, gene expression and
for screening positive clones.
Key
words:
Enhanced green fluorescent protein, gene expression, liver
specific vector, screening. |
