Full Length Research Paper

Botanical and molecular evidences of landraces from the germplasm exclusively collected from Baluchistan, a centre of diversity for Lens culinaris

Tayyaba Sultana¹ and  Abdul Ghafoor²*

¹GC University, Faisalabad, Pakistan.

²Plant Genetic Resources Program, Institute of Agricultural Biotechnology and Genetic Resources, National Agricultural Research Centre, Islamabad, Pakistan.

*Corresponding author. E-mail: ghafoor59pk@yahoo.com.

Accepted 15 June, 2009

   Abstract

Botanical descriptors, total seed proteins, isozymes and RAPD markers were applied to identify landraces from indigenous lentil germplasm exclusively collected from the province of Baluchistan, Pakistan. The Germplasm revealed the prevalence of landraces, especially on the basis of isozymes and RAPD markers. Dversity explored through various techniques revealed validity irrespective of the sample size from a particular district, RAPD being the best choice for investigating both inter and intra-accession variation that is needed to extend to more germplasm study along with botanical descriptors.

Key words: DNA marker, genetic resources, isozymes, lentil, SDS-PAGE, RAPD markers.