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Full Length Research Paper
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Mapping of QTLs for frost
tolerance and heading time using SSR markers in bread wheat
Omid Sofalian1*,
Seyyed A. Mohammadi2, Saeid Aharizad2,
Mohammad Moghaddam2 and Mohammad R. Shakiba2
1Department
of Agronomy and Plant Breeding, Faculty of Agriculture,
University of Mohaghegh Ardabili, Ardabil, Iran 179.
2Department
of Agronomy and Plant Breeding, Faculty of Agriculture,
University of Tabriz, Tabriz, Iran 51664.
*Corresponding author. E-mail:
sofalian@gmail.com.
Accepted
11 April, 2008 |
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Abstract |
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Selection
for complex genetic traits, such as frost tolerance, can be
simplified in plant breeding programs when linked markers
were detected. The use of microsatellite markers for tagging
and mapping important genes or QTLs is a goal in wheat
genetic projects. In this study, 200 microsatellite markers
were studied and after parental assay, 41 polymorphic SSR
markers were used for genotyping of 280 F2 individual plants
derived from F1 generation of wheat cross (Norstar× Zagros).
The progeny of individual F2 plants were used as F2:3
families for the assessment of LT50 and heading time. Single
marker analysis revealed that seven markers with total of
27% of phenotypic variance determination linked to LT50 and
five markers linked to the heading time. Two markers that
were located on 2B and 5A chromosomes affected both LT50 and
heading time significantly. It was assumed, therefore, that
some closely linked QTLs or QTLs with pleiotropic effects
govern both traits simultaneously, as the LT50 of F2:3
families were significantly correlated with the heading time
of F2:3 families, Thus, it is concluded that later heading
time is associated with the higher level of frost tolerance
in wheat.
Key
words:
Bread wheat, frost tolerance, heading time, QTL mapping,
single marker analysis, SSR. |
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